VTPB 212: Genetics in the News
Hours: 3 credits
Offered: Fall and spring
Summary: Examination of contemporary news articles from the popular press for class discussion of the underlying genetics and genomics research and their methodologies to gain a deeper understanding of the science behind news headlines in this increasingly important field.
Prerequisites: Freshman/Sophomore classification or approval of instructor; high school or college biology course recommended.
Hours: 3 credits
Offered: Fall and spring
Summary: Examination of contemporary news articles from the popular press for class discussion of the underlying genetics and genomics research and their methodologies to gain a deeper understanding of the science behind news headlines in this increasingly important field.
Prerequisites: Freshman/Sophomore classification or approval of instructor; high school or college biology course recommended.
GENE 608: Genetic Model Systems
Hours: 2 credits
Offered: Fall
Summary: Introduction to the main eukaryotic genetic model systems: yeast, C. elegans, Arabidopsis, Drosophila, zebrafish, and mouse through classical and contemporary literature.
Prerequisites: Graduate classification.
Hours: 2 credits
Offered: Fall
Summary: Introduction to the main eukaryotic genetic model systems: yeast, C. elegans, Arabidopsis, Drosophila, zebrafish, and mouse through classical and contemporary literature.
Prerequisites: Graduate classification.
Workshop on Techniques in Modeling Human Cancer in Mice
Location: The Jackson Laboratory, Bar Harbor, ME
Offered: Summer
Summary: This workshop provides training in the use of genetically defined laboratory mice as tools for asking questions about gene function and the role of genetics in the biology of cancer. Lectures will be given in the morning, followed by intensive hands-on laboratory sessions. Didactic lectures will reflect current knowledge of modeling cancer in a variety of organ sites and will emphasize common themes in cancer research and modeling. During the hands-on laboratory sessions, participants will develop skills in cancer model manipulations and surgical techniques that they will be able to apply to their own research. The techniques learned are broadly applicable to many cancer types, such as brain, breast, colon, leukemia/lymphoma, and prostate cancer. Participants completing the course will acquire a practical knowledge of how to characterize and analyze specific mouse cancer models.
Prerequisites: Post-baccalaureate classification.
Location: The Jackson Laboratory, Bar Harbor, ME
Offered: Summer
Summary: This workshop provides training in the use of genetically defined laboratory mice as tools for asking questions about gene function and the role of genetics in the biology of cancer. Lectures will be given in the morning, followed by intensive hands-on laboratory sessions. Didactic lectures will reflect current knowledge of modeling cancer in a variety of organ sites and will emphasize common themes in cancer research and modeling. During the hands-on laboratory sessions, participants will develop skills in cancer model manipulations and surgical techniques that they will be able to apply to their own research. The techniques learned are broadly applicable to many cancer types, such as brain, breast, colon, leukemia/lymphoma, and prostate cancer. Participants completing the course will acquire a practical knowledge of how to characterize and analyze specific mouse cancer models.
Prerequisites: Post-baccalaureate classification.
Mouse Development, Stem Cells & Cancer
Location: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Offered: Summer
Summary: Intensive lecture and laboratory course designed for scientists interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology. The laboratory and workshop portions of the course provides hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. Experimental techniques include genome editing by CRISPR/Cas9, pronuclear microinjection, isolation and culture/manipulation of pre- and post-implantation embryos, embryo transfer, embryo electroporation and roller bottle culture, chimera generation, generation and differentiation of mouse embryonic stem cells as well as induced pluripotent stem cells and fibroblasts, vibratome and cryosectioning, in situ RNA hybridization, immunostaining, FACS sorting and analysis, vascular injections and casting, neural sterotaxic injections, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy.
Prerequisites: Post-baccalaureate classification.
Location: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Offered: Summer
Summary: Intensive lecture and laboratory course designed for scientists interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology. The laboratory and workshop portions of the course provides hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. Experimental techniques include genome editing by CRISPR/Cas9, pronuclear microinjection, isolation and culture/manipulation of pre- and post-implantation embryos, embryo transfer, embryo electroporation and roller bottle culture, chimera generation, generation and differentiation of mouse embryonic stem cells as well as induced pluripotent stem cells and fibroblasts, vibratome and cryosectioning, in situ RNA hybridization, immunostaining, FACS sorting and analysis, vascular injections and casting, neural sterotaxic injections, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy.
Prerequisites: Post-baccalaureate classification.